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Sunday, July 12, 2020 | History

2 edition of Protocol for measuring in vitro steroid production by fish gonadal tissue found in the catalog.

Protocol for measuring in vitro steroid production by fish gonadal tissue

Great Lakes Laboratory for Fisheries and Aquatic Sciences.

Protocol for measuring in vitro steroid production by fish gonadal tissue

by Great Lakes Laboratory for Fisheries and Aquatic Sciences.

  • 128 Want to read
  • 40 Currently reading

Published by Great Lakes Laboratory for Fisheries and Aquatic Sciences in Burlington, Ont .
Written in English

    Subjects:
  • Ecology.,
  • Toxicology.,
  • Fishes -- Reproduction.

  • Edition Notes

    Statementby M.E. McMaster ... [et al.].
    SeriesCanadian technical report of fisheries and aquatic sciences -- 1961
    ContributionsMcMaster, M. E.
    Classifications
    LC ClassificationsSH"223"C355"no.1961
    The Physical Object
    Paginationviii, 78 p. :
    Number of Pages78
    ID Numbers
    Open LibraryOL20452424M

    In vitro digestibility analysis made easier with the Gas Endeavour. Animal feed & nutrition - in vitro analysis If you are working in research and development on animal feed, diet formulation, nutrients or supplements for ruminants, you can rely on Bioprocess Control’s Gas Endeavour to provide accurate and precise volumetric gas production. For In Vitro Diagnostic Use Directions: 1. Specimen should be thoroughly fixed before decalcification. Most standard fixatives can be used prior to RDO GOLD use. To insure adequate fixation and decalcification, specimens should be trimmed to less than 1 cm thickness. Formalin fixation and RDO GOLD decalcification should not be combined.

    Production of reproductively sterile fish by a non-transgenic gene silencing technology Ten-Tsao Wong & Yonathan Zohar We developed a novel bath-immersion technology to produce large numbers of infertile fish. As seafood consumption shifts from fishery harvests towards artificially propagated species,File Size: KB. Artificial testis to study early gonadal development and germ cell differentiation we aimed in this thesis at studying the early development and differentiation of the male germ cells in vitro, in order to find a robust protocol for fertility preservation for prepubertal male patients. Author: Ahmed Reda.

    Gonadal steroids, selective serotonin reuptake inhibitors, and mood disorders in women. Med Clin North Am. ; 87(5) (ISSN: ) Huttner RP; Shepherd JE. That gonadal steroids influence the limbic system and that they affect neurotransmitter activity is undisputed. An in vitro assay was developed to measure the Chemother apeutic drug susceptibility of cells from human tumors. The assay utilized live cells, freshly isolated from tumor tissue, which were incubated for a short period in vitro. The drug-induced inhibition of incorporation of radiolabeled precursor into DNA, RNA, and protein was measured. The File Size: KB.


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Protocol for measuring in vitro steroid production by fish gonadal tissue by Great Lakes Laboratory for Fisheries and Aquatic Sciences. Download PDF EPUB FB2

Abstract. The initial clinical, pathological, and hormonal investigation of a patient with testicular feminization syndrome is described. Incubation of gonadal tissue with various radioactive substrates, together with the isolation and identification of the resulting metabolites, was demonstrated a high capacity to synthesize : E.

Lockwood, P. Ghosh, G. Pennington, R. Tipton. GONADAL STEROID METABOLISM IN Coris DISCUSSION From the above-mentioned data, it is apparent that the pattern of gonadal steroid metabolism in the protogynous wrasse C.

julis fits well into the current knowledge on the biosynthetic activities of ovarian and testicular tissue from gonochoristic species (e.g., Ozon, a, b; Kime, Cited by: In both sexes, significant depressions in circulating testosterone and in in vitro gonadal testosterone production were associated with exposure to whole condensates, particulate extracts, one SPE.

The initial clinical, pathological, and hormonal investigation of a patient with testicular feminization syndrome is described. Incubation of gonadal tissue with various radioactive substrates, together with the isolation and identification of the resulting metabolites, was demonstrated a high capacity to synthesize testosterone.

Two biosynthetic pathways were demonstrated, originating from Author: E. Lockwood, P. Ghosh, G. Pennington, R. Tipton. The ovotestis was removed and incubated in vitro with tritiated steroids (testosterone, dehydroepiandrosterone, pregnenolone and 17 alpha-hydroxyprogesterone).

Labeled metabolites were isolated and identified. Based upon these findings, a pathway of steroid biogenesis in this abnormal gonadal tissue is : T. Vancrombreucq, M. Govaerts, Pierre Preumont, Jean Schwers. The ability of ovarian tissue to synthesize putative maturational steroids was assessed by measuring production of 17α,20β-dihydroxypregnen- 3-one (17,20βP) by ovarian fragments incubated.

GENERAL AND COMPARATIVE ENDOCRINOL () Histology, Ultrastructure, and in Vitro Steroidogenesis of the Testes of Two Male Phenotypes of the Protogynous Fish, Thalassoma duperrey (Labridae) THOMAS F. HOURIGAN,*'t MASARU NAKAMURA,t YOSHITAKA NAGAHAMA,* KOHEI YAMAUCHI,AND E.

GORDON GRAUt *Laboratory of Reproductive Biology, National Institute Cited by: Teleost fish produce several types of bioactive gonadal steroids, including estrogens, progestogens, androgens and numerous other steroids.

Bioactive steroids are produced in specialized cells within the ovarian follicle (theca, granulosa) and the testis (Leydig cells). The present study was performed to determine the in vitro steroidogenic capacity of a gonadal sample from a patient suffering from a complete androgen resistance syndrome.

Testosterone and estradiol production by the testicular tissue from this patient as well as gonadotropin binding to a membrane fraction prepared from this tissue were measured.

hCG bound with high affinity but with a Cited by: 3. In vitro ketotestosterone and 17α,20β-dihydroxypregnenone production by testicular fragments and isolated sperm of rainbow trout, Salmo gairdneri.

by: The concept of the production rate of a steroid hormone was introduced to describe the rate at which the hormone enters the circulation de novo, regardless of its origin. Therefore, by definition, the production rate of a steroid hormone is equal to the glandular secretion rate of the hormone plus the secretion rates of any other steroids that.

In order to clarify the basic questions with regard to sex change in the protandrous anemonefish Amphiprion frenatus, gonadal structure of females and males, distribution and activity of steroid producing cells and the ability of gonads to produce sex hormones were fish out of 29 had ovaries with many previtellogenic and vitellogenic oocytes and an ovarian cavity, but they have Cited by: Fish were caught in the inland waters of the Reloncaví Sound, Interior Sea of Chiloé, Chile.

Samples of gonads and blood were collected for histology and sex steroid hormone (17 β-estradiol, ketotestosterone 20 β-dihydroxypregnenone) analysis, respectively.

Sex steroid hormone quantification was performed usingAuthor: Manuel Alvarado, Edison Serrano, Juan Carlos Sánchez, Luis Valladares. The present study concerns the in vitro production of C18 steroids from endogenous precursors by an accessory reproductive organ of this animal, the bursa copulatrix or spermatheca by:   This assay was designed to detect enzyme inhibition in the steroid biosynthesis pathway by measuring decreased testosterone production; however, the assay was removed from consideration because of high false-positive and false-negative responses and difficulty assessing Leydig cell cytotoxicity in the mixed population of cells present in minced Cited by: Browse our database of protocols for cell culture, nucleic acid, protein analysis, RNA and DNA applications, cloning, cell analysis, and drug discovery research.

The research also involved the development and testing of an in-vitro fish liver S9 biotransformation testing method. The results show that biotransformation rates using fish liver fractions are highly dependent on the concentration of the test chemical in the test. As a result, the recommended 1 µM initial substrate concentration mayAuthor: Justin Lo.

Gonadal modulation of in vitro steroidogenic properties of dispersed adrenocortical cells from Sceloporus lizards Rocco V. Carsiaa, Patrick J. McIlroyb, Robert M. Coxc, Michele Barrettd, Henry B. John-Aldere,* a Department of Cell Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJUSA bDepartment of Biology, Camden College of Arts.

OECD Guidance Document for the Diagnosis of Endocrine-related Histopathology of Fish Gonads Page 5/96 Summary Exposure of fathead minnow (Pimephales promelas), Japanese medaka (Oryzias latipes) and zebrafish (Danio rerio) to endocrine disrupting chemicals may result in histopathological altera-tions of gonadal structure and Size: 2MB.

The need for in vitro test for endocrine disruption 18 Test for endocrine disruptors in vitro 18 In vitro receptor binding assays 20 Induction of hormone responsive mammalian cell proliferation 21 Transactivation assays (hormone-sensitive transcription of reporter genes).

When aromatization was intact, fat accumulation began with mild gonadal steroid deficiency (a testosterone level of approximately to ng per deciliter), whereas lean mass, thigh-muscle area Cited by:   The honeycomb grouper shows protogynous hermaphroditism.

The endocrine mechanisms involved in gonadal restructuring throughout protogynous sex change are largely unknown. In the present study, we investigated changes in the gonadal structures and levels of serum sex steroid hormones during female to male sex change in the honeycomb grouper.

On the basis of histological Cited by: cific microbiota in the secretion of gonadal steroid hormones in the developmental stage, we investigated the change in fecal hormone levels in 4‐week‐old GF mice orally administered the fecal microbi‐ ota of SPF mice or guinea pigs (GF‐SPF mice or GF‐guinea pig mice).

In addition, we also conducted odor preference test with 8‐week Cited by: 4.